Studies on the Effects of L-Prolinamide, 5-OXO-L-Prolyl-L-Phenylanyl-4-Hydroxy Compound Produced by Pseudomonas Fluorescence against Cell Wall Protein (3GNU Receptor) of Pythium SPP MTCC 10247

Abstract

The present study was carried out inorder to assess the biocontrol efficiency of Pseudomonas fluorescence against Pythium spp MTCC 10247.  The earlier studies have revealed its antifungal properties but the previous reports lack the details about the compounds and the target for the fungi. Hence we have designed our work plan to reveal the compounds present in the secondary metabolites of Pseudomonas fluorescence and the target on the phytopathogens Pythium spp MTCC 10247 which causes plant diseases. Production of secondary metabolites of     Pseudomonas fluorescence was carried out and the compounds were characterized by TLC, SDS-PAGE, and GC-MS. There were 7 peak compounds found out,  they are 1,4-diaza-2,5-dioxobicyclo[4.3.0] nonane (13,69%) , 3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione(10.11%), Pyrrolo[1,2-a] pyrazine 1,4dione,hexahydro-3-(2-methylpropyl(17.79%) l-Leucine,N-cyclopylcarbonyl-pentadecyl ester(7.09%), 3,6diisobutyl-2,5-piperazinedione (37.62%), 3-benzylhexahydropyrrolo[1,2-A]pyrazine-1,4-dione(8.52%), L-prolinamide, 5-oxo-l-prolyl-l-phenylanyl-4-hydroxy(5.19%). Antifungal activity of the ethyl acetate extract of the secondary metabolites were examined against Pythium spp MTCC 10247 and result revealed that the lag period of  the fungi was double fold higher than the control and the compounds were fungi static because there was a 57% of growth reduction after 7^th day of incubation period.    All the seven compounds were used for molecular docking study and result showed higher score value with L-prolinamide, 5-oxo-l-prolyl-l-phenylanyl-4-hydroxy against cell wall protein (3GNU receptor) Pythium spp MTCC 10247.