The rhizosphere region is rich with the plenty of microorganisms. It may be either pathogenic or non-pathogenic to the plants and animals. Fungi were the ubiquitous organisms present in all types of habitats (Ainsworth  et al., 1995).They play a vital role in conversion of waste and degradation (Diana 1994).. There were the previous researchers so far explained more than million fungal species on earth (Barnett et al., 1972). The physcio-chemical parameter was also analyzed. The soil samples were serially diluted and inocualted on the SDA plates. The different species belongs to various groups of fungi such as Ascomycotina, Zygomycotina and Deuteromycotina were identified with the help of relevant literatures. A total of -- taxa belonging to -- genera were isolated, these include one species of Acomycetes, one species of Coelomycetes five species of Zygomycetes and remaining species were Deuteromycetes. Penicillium and species of Aspergillus were also recorded from samples. Basidiomycetes were also identified.  The macro nutrients such as N, P and K content were not rich in the samples. There is no proper record so far regarding the fungal in these soil areas along with the soil nutrients. The present study is mainly focused on the mycoflora present in the rhizosphere regions of three different soil of puducherry region based on the macro and micronutrients present in the soil.