Peer Reviewed Open Access

This paper is reviewed in accordance with the Peer Review Program of IRA Academico Research

Occurrence of SHV GENE and Antibiogram of Klebsiella Pneumoniae Infection in Swiss Albino Mice Colony

M. Thangapandiyan, S. P. Preetha, S. Suresh Kannan, T. Mohanapriya
DOI: http://dx.doi.org/10.21013/jas.v3.n3.p18
Abstract

An epizootic causing high morbidity and high mortality in Swiss albino inbred mice over a period of two weeks caused by Klebsiella pneumoniae is reported. Diagnosis was based on clinical signs, histopathological, bacteriological evaluation of the lung, kidney and intestinal tissue and multiplex PCR. Biochemical tests were positive for citrate, nitrate, urease, lysine decarboxylase, ornithine decarboxylase and negative for phenylalanine and hydrogen sulphide production. Carbohydrates viz., glucose, lactose, adonitol, arabinose were fermented but sorbitol was not. Disc diffusion tests for antibiotic sensitivity showed susceptibility to enrofloxacin, amikacin, doxycyline and resistant to cephalexin, cefadroxil, cefuroxime, cephtriaxone and ampicillin. Amplification by multiplex PCR for sulphydryl variable (SHV) gene yielded a product size of 472bp.

Keywords
Mice, Klebsiella sp, pneumonia, antibiogram
Full Text:
PDF

©IRA Academico Research & its authors
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. This article can be used for non-commercial purposes. Mentioning of the publication source is mandatory while referring this article in any future works.