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Induction of Herbicide resistance via seed-derived rice (Oryza sativa) Calli

E. M. S. I. Ekanayaka, S. R. Weerakoon, T. D. Silva, S. Somaratne
DOI: http://dx.doi.org/10.21013/jas.v3.n3.p15
Abstract

Herbicide resistant (HR) rice improves efficiency of weed management and HR varieties can develop through induced mutagenesis. The present investigation was focused on mutagenic response of seed-derived rice calli to different EMS concentrations and evaluation for HR. Seed-derived calli were obtained from glyphosate-susceptible rice variety (Bg250) and exposed to varying  concentrations of EMS  (0.1%, 0.2%, 0.3% and 0.4%). EMS treated calli were exposed to glyphosate (0.2%) and Tetrazolium test (1% TTC) was applied to identify cell viability in calli. Fluorescent AFLP analysis was performed on EMS treated calli.  Results indicated that mutation of calli was higher at EMS concentration range of 0.1 – 0.2%.  Bg250 calli demonstrated resistance to glyphosate at 0.2%. FAFLP analysis revealed E11M32 marker was found to be a specific marker to identify the induced HR in rice. The results of the study revealed that in vitro application of EMS on callus has ability to develop HR rice calli. 

Keywords
EMS, Glyphosate, Seed culture, AFLP
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