Bacterial Diversity Analysis of Upper Lake, Bhopal (M.P) India, Using Metagenomics

Abstract

Diversity analysis in reference to current methodology offers advantage over existing methodology such as biochemical characterization etc. Present study is carried out in order to establish diversity pattern using metagenomic procedure. The water body situated in Bhopal has its own recreation and water supply potential. Any water body is entertained by biological activity preferably predominant in sediment, as sediment represent site of various biological and geochemical activity. The protocols combine the application of mechanical (Beads and Sonicator ) and soft lysis (SDS and enzyme) method for the isolation of total DNA from sediment of Upper lake of Bhopal followed by its quantification and purity assessment. The quality and purity of metagenomic DNA was quite consistent and reliable, although it contained residual concentration of humic acid. The methods have wide applicability in investigating molecular diversity and exploring functional genes.
The methodology used showed supportive observation of sediment habitat as use of extracted DNA when amplified using 16s rDNA region. Phylogenetic relationship was established. Dendogram was prepared using NTSYS ( software for clustering of the data) which is based on unweighted Pair Group Method with Arithmetic Averaging (UPGMA). The dendogram showed deviation of initial two groups at 27% as well as 42% similarity. Sample B1 and B13 from Boat club site falls with absolute similarity, B4 and B10 also shows absolute resemblance in relation to pattern of amplification.